Journal: Lab on a Chip

Article Title: A microfluidic rheometer for tumor mechanics and invasion studies

doi: 10.1039/d5lc00504c

Figure Lengend Snippet: Strain measurement and the strain relaxation time of the spheroids. A1. Schematic of a tumor spheroid before (yellow, dashed lines) and during (red, solid lines) compression. The piston moves down vertically compressing a tumor spheroid underneath, resulting in the change in cross-sectional radius of the tumor spheroid. A2. A micrograph of an uncompressed MCF-10A tumor spheroid imaged at vertical midplane. The yellow outline indicates the area of the uncompressed spheroid A o , and the redline indicates the area of the compressed spheroid, A c . The scale bar is 100 μm. B. Spheroid strain response to sinusoidal, square, and triangular pressure wave compression with a period of 20 seconds from the microrheometer. The blue dots show the strain of the tumor spheroid obtained from bright field images with respect to time and the red dots represent the pressure applied to the pressure controller. The sampling rate is 15.67 Hz. C. Strain response of MDA-MB-231 and MCF-10A tumor spheroids when subjected to square wave compression. The maximum pressure here is 10 kPa. D. Half-relaxation time of the MDA-MB-231 and MCF-10A spheroids. Half relaxation time was defined as the time it takes for the strain to decrease to 50% of its original value after the pressure is released.

Article Snippet: Then, a tubing (OD and ID of 1/16′′ and 1/32′′, respectively) pre-filled with water from the pressure controller (Elveflow OB1 MK4, Paris, France) was connected to the 1.5 mm inlet on the device.

Techniques: Sampling